Coding

Part:BBa_K4359007:Design

Designed by: Ira Zibbu   Group: iGEM22_IISER_TVM   (2022-10-07)


V5 Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was generated from reverse translating the amino sequence GKPIPNPLLGLDST, with codons optimized for E.coli


Source

The V5 tag derived from the P and V protein of the simian virus 5. In particular, it is amino acid residues 95 to 108 of RNA polymerase alpha subunit.

References

[1] https://www.ptglab.com/news/blog/v5-tag-properties/